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KMID : 0381120220440101191
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Genes and Genomics
2022 Volume.44 No. 10 p.1191 ~ p.1199
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CDK1 promotes the proliferation of melanocytes in Rex rabbits
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Dai Yingying
Hu Shuaishuai
Bai Shaocheng
Li Jiali
Yang Naisu
Zhai Pin
Zhao Bohao
Chen Yang
Wu Xinsheng
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Abstract
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Background: The fur color constitutes one of the most important economic characteristics of fur animals and is determined by the content of melanin. A previous study has shown that the cyclin-dependent kinase 1 (CDK1) is a member of the protein kinase family, involved in forming the color of the fur in Rex rabbits. However, its effect on the melanocytes remains unclear.
Objective: This study aimed to provide evidence for the role of CDK1 in melanogenesis.
Methods: This study measured the expression of CDK1 in Rex rabbit skins of six coat colors using qRT-PCR. The CDK1-mediated regulation of the pigmentation-related genes and cyclin-dependent kinases were analyzed. The melanin content, proliferation, and apoptosis of the melanocytes were analyzed using the NaOH, CCK8, and Annexin V-FITC methods.
Results: The CDK1 expression in the skin of the rex rabbits with different coat colors was found to be regular, and the expression level was found to be the highest in the skin of the black rex rabbits (P?
Conclusions: The results indicated that CDK1 can serve as a key gene regulating melanogenesis, melanocyte proliferation, and apoptosis, providing a new theoretical basis for studying the mechanism by which the different colors of the fur evolve in mammals.
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KEYWORD
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CDK1, Rex rabbit, Melanocyte, Melanin deposition
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